Cryopreservation can be done by
· Slow freezing
· Vitrification (fast freezing): this is the best and most used way to preserve sperm, eggs and embryos. The survival rates for the cells frozen with this technique are high, with good outcomes .
Semen samples are collected and checked under a microscope to count sperm cells and check if they are healthy. These sperm cells are then frozen and stored for future purposes.
Egg freezing requires treatment similar to IVF and has multiple steps including ovarian stimulation, egg retrieval, and freezing.
· Ovarian stimulation is the first step to produce multiple eggs
· These eggs are later retrieved out of the woman's body by a vaginal procedure known as egg retrieval.
· Finally, the retrieved eggs are frozen by a process known as vitrification. This process helps to preserve the eggs in a stable condition for future use
Once the eggs are available as above, they can be fertilised with sperm by the process of IVF or ICSI, to make the embryos Day 2-5 embryos can be then subject to vitrification technique (fast freezing) and stored for longer periods of time. Ovarian tissue:
Ovarian tissue can also be taken out and frozen for very young girls or women in need of urgent start to a cancer treatment.